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0 (hodnocen0 x )

BK

New York : Humana Press, [2018]

xvi, 375 stran : ilustrace (některé barevné) ; 27 cm

ISBN 978-1-4939-7308-8 (vázáno)

Methods in molecular biology, ISSN 1064-3745 ; 1673

Springer protocols

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Contents // Foreword... v // Preface... vii // Contributors... xiii // Part I Detection and Quantification of Quorum Sensing Signal Molecules // 1 Use of Whole-Cell Bioassays for Screening Quorum Signaling, // Quorum Interference, and Biofilm Dispersion... 3 // Starla G. Thornhill and Robert J.C. McLean // 2 Detection of 2-Alkyl-4-Quinolones Using Biosensors... 25 // Matthew R Fletcher, Stephen R Dijjgle, Miguel Camara, and Paul Williams // 3 “Hot Stuff’: The Many Uses of a Radiolabel Assay // in Detecting Acyl-Homoserine Lactone Quorum-Sensing Signals... 35 // Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg // 4 Liquid Chromatography/Mass Spectrometry (LC/MS) // for the Detection and Quantification of N-Acyl-L-Homoserine // Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)... 49 // Francois Lepine, Sylvain Milot, Marie-Christine Groleau, and Eric Déziel // 5 Detection of the Bacterial Quorum-Sensing Signaling Molecules N-Acyl-Homoserine Lactones (HSL) // and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass // Spectrometry (UHPLC-MS)... 61 // Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, and Anton Hartmann // 6 Biosensors for the Detection and Quantification of AI-2 Class // Quorum-Sensing Compounds... 73 // Sathish Rajamani and Richard Sayre // 7 Detection of Agr-??? Autoinducing Peptides Produced // by Staphylococcus aureus... 89

// Ewan J. Murray and Paul Williams // 8 Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal // Factor (DSF) Family Quorum-Sensing Signals... 97 // Lian Zhou, Shuang Sun, Wei Zhang, and ??-Wen He // 1 // x Contents // 9 Rapid Electrochemical Detection of Pseudomonas aeruginosa // Signaling Molecules by Boron-Doped Diamond Electrode... 107 // Alyah Buzid, John H.T Luong, F. Jerry Keen, Fergal ?’Gara, // Jeremy D. Glennon, and Gerard R McGlacken // 10 Detection and Quantification of Butyrolactones from Streptomyces... 117 // Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano // Part II Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level // 11 Fluorescence Quenching Studies of y-Butyrolactone-Binding // Protein (CprB) from Streptomyces coelicolor ?.?(2)... 131 // Jessy Mariam and Ruchi Anand // 12 Methods to Study Solo/Orphan Quorum-Sensing Receptors... 145 // Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, // and Brian M.M. Ahmer // 13 Enzymatic Assays to Investigate Acyl-Homoserine Lactone // Autoinducer Synthases... 161 // Daniel Shin and Rajesh Nagarajan // 14 Global Expression Analysis of Quorum Sensing-Controlled // Genes by RNAseq... 177 // Charlotte D. Majerczyk // 15 Identification of AHL- and BDSF-Controlled Proteins // in Burkholderia cenocepacia by Proteomics... 193 // TTilei Liu, Gabriella Pessi, Katharina Riedel, and Leo Eberl // 16 Imaging

AT-Acyl Homoserine Lactone Quorum Sensing In Vivo... 203 // Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, // Michael Givskov, and Thomas Bjarnsholt // 17 Assessing Pseudomonas aeruginosa Autoinducer Effects // on Mammalian Epithelial Cells... 213 // Jake Everett, Rebecca Gabrilska, Kendra R Rumbaugh, and Elena Vikström // 18 Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies... 227 // Damien Maura, Arunava Bandyopadhaya, and Laurence G. Rahme // 19 Methods to Study Quorum Sensing-Dependent Virulence // and Movement of Phytopathogens In Planta... 243 // Shulamit Manulis-Sasson and Laura Chalupowicz // 20 Differential Equations Models to Study Quorum Sensing... 253 // Judith Perez-Veldzquez and Burkhard A. Hense // Part III Identification and Characterization of Anti-Quorum Sensing Agents // 21 Qualitative and Quantitative Determination of Quorum Sensing // Inhibition In Vitro... 275 // Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist, // Thomas Bjarnsholt, and Michael Givskov // Contents xi // 22 A Coculture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum // Sensing Inhibitors... 287 // Giordano Rampioni, Giulia Giallonardi, Francesca D’Angelo, and Livia Leoni // 23 A Culture-Dependent Method for the Identification of Quorum // Quenching Enzymes of Microbial Origin... 297 // Kaihao Tang and Xiao-Hua Zhang // 24 Directed Evolution of Quorum-Quenching Enzymes: A Method for the Construction of a Directed

Evolution Platform // and Characterization of a Quorum-Quenching Lactonase // from Geobacillus kaustophilus... 311 // Maybelle Kho Go, Jeng Teong Chow, and Wen Shan Tew // 25 Generation of High-Sensitivity Monoclonal Antibodies Specific // for Homoserine Lactones... 325 // Soumya Palliyil // 26 Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi // Through Structure-Based Virtual Screening... 353 // Tianyu Jiang, Peng Zhu, Lupei Du, and Minyong Li // 27 Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of ö-Hemolysin with High Performance // Liquid Chromatography... 363 // Cassandra L. Quave and Alexander R. Horswill // Erratum to: Fluorescence Quenching Studies of y-Butyrolactone-Binding // Protein (CprB) from Streptomyces coelicolor ?.?{2)... El // Index... 371