Part 1. Introduction to the CRISPR Revolution.- 1. Viral Vectors, Engineered Cells and the CRISPR Revolution -- 2. Combining Engineered Nucleases with Adeno-associated Viral Vectors for Therapeutic Gene Editing -- Part 2. CRISPR in Model Systems -- 3. From Reductionism to Holism: Toward a More Complete View of Development through Genome Engineering -- 4. Genome Engineering in Mice -- 5. Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors -- 6. From Prokaryotic Immune Systems to Plant Genome Editing Tools -- 7. Part 3. The Future of CRISPR -- 7. Using High-throughput Gene Editing to Identify Therapeutic Targets in the Noncoding Genome -- 8. CRISPR in the Retina: Evaluation of Future Potential -- 9. The Future of CRISPR Applications in the Lab, the Clinic and Society.
This book presents descriptive overviews of gene editing strategies across multiple species while also offering in-depth insight on complex cases of application in the field of tissue engineering and regenerative medicine. Chapters feature contributions from leaders in stem cell therapy and biology, providing a comprehensive view of the application of gene therapy in numerous fields with an emphasis on ophthalmology, stem cells, and agriculture. The book also highlights recent major technological advances, including ZFN, TALEN, and CRISPR. Precision Medicine, CRISPR, and Genome Engineering is part of the highly successful Advances in Experimental Medicine and Biology series. It is an indispensable resource for researchers and students in genetics as well as clinicians. ..