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Bibliografická citace

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EB
EB
ONLINE
1. elektronické vydání
Karolinum 2018
1 online zdroj (136 stran)
Externí odkaz    Plný text PDF (Bookport) 
   * Návod pro Bookport 


ISBN 978-80-246-2544-7 (online ; pdf)
ISBN 978-80-246-2413-6 (print)
Tištěná verze: Melter, Oto. Principles and practicals in medical microbiology. [Place of publication not identified] : Karolinum Press, c2014 ISBN 978-80-246-2413-6
The book has been created for medical students to ease the comprehension of the relations between theory and practice in current medical microbiology. The core comprehensive data are reviewed from prestigious publications in the field and professional experience of the authors. Each chapter contains a theoretical and a practical part. The practical part is divided into a few exercises which allows for the practice of some of the basic experimental procedures used in laboratories of medical microbiology. We believe that the original photographs and the diagrams that have been newly created by the authors will improve the understanding of the basic principles of microbiological diagnosis. Each chapter is complemented with a lab quiz intended to help students review their basic knowledge..
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CONTENTS // Introduction ... // I. Laboratory safety rules... // 1.1 Purpose of the safety principles // 1.2 Principles... // GENERAL MICROBIOLOGY // 2. // Specimen collection & diagnostic principles // 2.1 Specimen collection & transport... // 2.2 Material & methods... // 2.3 Conditions for specimen collection & transport ... // 2.4 Request form ... // 2.5 Diagnostic principles in medical microbiology... // 2.6 Light microscopy... // 2.7 Gram staining basic staining procedure in clinical microbiology... // 2.7.1 Smear preparation... // 2.7.2 Quality of cell wall & Gram staining procedure... // 2.8 Practical part specimen collection & diagnostic principles ... // 2.9 Lab quiz...?... // 3. Direct detection & typing of infectious agents ... // 3.1 Definition of agent detection & typing ... // 3.2 Application of microscopy technics... // 3.3 Application of nucleic acid based methods... // 3.3.1 Principle of DNA amplification by POR methods ... // 3.3.2 Principle of DNA hybridization methods... // 3.3.3 Principle of sequence based methods... // 3.4 Other methods in direct antigen detection... // 3.4.1 Immunochromatographic method... // 3.5 Practical part - direct detection & typing of infectious agents... // 3.6 Lab quiz... // 4. Cultivation of infectious agents ... // 4.1 Cultivation - definition ... // 4.2 Cultivation ofbactcrial. viral & parasitic agents... // 4.3 Media for bacterial cultivation... // 4.4 Examples ofbactcrial cultures... // 4.5 Conditions for cultivation ...
4.6 Special staining procedures... // 9 // II // II // II // 13 // 13 // 13 // 14 // 14 // 15 // 15 // 16 16 16 // 17 // 18 // 19 // 19 // 19 // 21 // 21 // 22 // 22 // 22 // 22 // 23 // 24 // 25 25 25 // Ł Ł S S // 4.7 Praclical pari - special stainings & cullivalion of infectious agents... 28 // 4.8 Lab quiz... 30 // 5. Antibiotic susceptibility testing ... 31 // 5.1 Antimicrobials: mode of action & resistance... 31 // 5.2 Antimicrobial susceptibility testing... 31 // 5.3 Basic methods ... 32 // 5.4 Interpretations of the results... 32 // 5.5 Other methods; procedures & interpretation... 33 // 5.6 Practical part special stainings «S: cultivation of infectious agents... 34 // 5.7 Lab quiz... 36 // 6. Identification of infectious agents... 37 // 6.1 IX’finilion of identification of infectious agents... 37 // 6.2 Phenotypical methods ... 37 // 6.3 Application of phenotypical methods ... 40 // 6.4 Cicnotypical methods & application... 41 // 6.5 Practical part - identification of infectious agents... 41 // 6.6 Ixibquiz... 43 // 7. Detection of specific antibodies... 45 // 7.1 Immune response... 45 // 7.2 Antigens & antibodies... 45 // 7.3 liasic serological methods... 46 // 7.4 Serological methods & application ... 48 // 7.5 Praclical pan - detection of specific antibodies... 49 // 7.6 Lab quiz... 50 // 8. General mycology ... 51 // 8.1 Fungi ... 51 // 8.2 Classification ... 51 // 8.3 Diagnostics... 52 // 8.3.1 Microscopy... 52 // 8.3.2 Cultivation... 53 // 8.3.3 Identification...
53 // 8.4 Susceptibility to antimycotical drugs... 56 // 8.5 Serological methods... 56 // 8.6 Direct detection of the agent in clinical material... 57 // 8.7 Practical pan general mycology... 57 // 8.8 Lab quiz... 58 // 9. General virology ... // 9.1 Definition of viruses... // 9.2 History... // 9.3 Klcctronmicroscopic evidence .. // 9.4 Virus detection by other methods // 9.5 Practical pan - general virology // 9.6 Lab quiz... // 10. General parasitology... // 10.1 Parasitism, parasite and host // 10.2 Classification ... // 10.3 Diagnostic methods... // 10.4 Material and diagnostics ... 67 // 10.5 Practical pan - general parasitology ... 68 // 10.6 Lab quiz...:... 69 // saa a sssssss // 11. Molecular microbiolog)’ & epidemiolog) // 11.1 // 11.2 // 11.3 // 11.4 // 11.5 // 11.6 // 11.7 // Definition... // Bacterial cell wall synthesis and antibiotics ... // Antibiotic resistance genes... // Regulation of structural genes for antibiotic resistance .. // lipidcmiologic typing... // Practical part molecular microbiology & epidemiology Lab qui/... // SPECIAL MICROBIOLOGY // 12. Staphylococci... // 12.1 General features... // 12.2 Virulence factors and pathogenesis... // 12.3 Infections... // 12.4 Treatment, prevention «& control... // 12.5 Uboratory diagnosis... // 12.6 Practical part staphylococci ... // 12.7 Lab qui/... // 13. Streptococci & enterococci... // 13.1 General features... // 13.2 Classification ... // 13.3 Virulence factors and pathogenesis... // 13.4 Infections and
poststreptococcal diseases // 13.5 Treatment, prevention & control... // 13.6 Laboratory diagnosis... // 13.7 Praclical part streptococci & enterococci // 13.8 Lab qui/... // 14. Coryncbacteria & listeria // 14.1 General features___ // 14.2 // 14.3 // 14.4 // 14.5 // 14.6 // 14.7 // Virulence factors & pathogenesis ... // Infections & epidemiology... // Treatment, prevention & control... // Laboratory diagnosis... // Practical part - coryncbacteria & listeria Lab qui/... // 15. Enterobacteria & enteric pathogens ... // 15.1 General features... // 15.2 Virulence factors & pathogenesis ... // 15.3 Infections & epidemiology... // 15.4 Treatment, prevention & control... // 15.5 Laboratory diagnosis... // 15.6 Practical pan enterobacteria and enteric pathogens // 15.7 Lab qui/... // 16. Anaerobic bacteria ... // 16.1 General features... // 16.2 Virulence factors & pathogenesis // 16.3 Infections & epidemiology... // 16.4 Treatment, prevention & control . // 16.5 Laboratory diagnosis... // 16.6 Praclical part anaerobic bacteria // 16.7 Lab qui/... // 71 // 71 // 71 // 72 // 72 // 73 // 74 // 75 // 77 // 77 // 77 // 77 // 78 78 80 81 // 83 // 83 // 83 // 83 // 84 // 84 // 85 // 87 // 88 // 89 // 89 // 89 // 90 90 90 92 // 94 // 95 95 // 95 // 96 96 96 98 100 // 101 // 101 // 101 // 102 // 102 // 102 // 104 // 106 // 17. Heuseria, Bordetella & Haemophilus... 107 // 17.1 General fcaJurcs... 107 // 17.2 Virulence facíors & palhogcncsis ... 107 // 17.3 Infcclions& epidemiology... 107
17.4 Treatment, prevenlion & control ... 108 // 17.5 Laboratory diagnosis... 108 // 17.6 Praclical pan Neisseria. Bordetella. Haemophilus... 110 // 17.7 Lab quiz... 112 // 18. Pseudomonas aeruginosa & other non-fermenters... 113 // 18.1 General features... 113 // 18.2 Virulence factors & pathogenesis ... 113 // 18.3 Infections & epidemiology... 114 // 18.4 Treatment, prevention & control ... 114 // 18.5 Laboratory diagnosis... 114 // 18.6 Practical part - pseudomonas and non-fermenters ... 116 // 18.7 Lab qui/... 118 // 19. Mycobacteria... ?9 // 19.1 General features... 119 // 19.2 Classification of mycobacteria... 119 // 19.3 Virulence factors & pathogenesis ... 119 // 19.4 Infcctioas & epidemiology... 120 // 19.5 Treatment, prevention & control ... 120 // 19.6 Laboratory diagnosis... 121 // 19.7 Practical part mycobacteria... 122 // 19.8 Lab qui/... 124 // 20. Yeasts... 125 // 20.1 General features... 125 // 20.2 Virulence factors & pathogenesis ... 125 // 20.3 Infections & epidemiology... 126 // 20.4 Treatment, prevention & control ... 126 // 20.5 Laboratory diagnosis... 126 // 20.6 Practical part yeasts... 128 // 20.7 Lab qui/... 130 // 21. Arthropods... 131 // 21.1 General properties ... 131 // 21.2 Classification ... 131 // 21.3 Pathogenesis... 131 // 21.4 Infccton caused or trasmitted by arthropods ... 131 // 21.5 Treatment and prevention ... 132 // 21.6 Laboratory diagnosis... 133 // 21.7 Practical part arthropods... 134 // 21.8 Labquiz... 135

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