.

0 (hodnocen0 x )

EB

EB

ONLINE

Amsterdam : Elsevier, 2022

1 online zdroj

ISBN 9780080931357 (e-book)

ISBN 9780444522344 (vázáno)

001931761

Preface ix // 1. Introduction 1 // 1.1 Short history of capillary gel electrophoresis 4 // 1.2 The capillary gel electrophoresis system 6 // 1.2.1 Basic setup 6 // 1.2.2 The separation capillary 8 // 1.2.3 Power supply 8 // 1.2.4 Injection 9 // 1.2.5 Detection 10 // 1.2.6 Data acquisition 11 // 1.3 Separation modes in gel-filled capillary columns 12 // 1.3.1 CGE for molecular sieving 13 // 1.3.2 Capillary gel isoelectric focusing 14 // 1.3.3 Capillary gel isotachophoresis 15 // 1.3.4 Capillary affinity gel electrophoresis 16 // 1.4 Comparison with slab gel electrophoresis 16 // References 18 // 2. Basic principles of capillary gel electrophoresis 21 // 2.1 The electrophoretic migration 21 // 2.2 Efficiency and resolution 27 // 2.3 Band broadening in capillary gel electrophoresis 29 // 2.4 Temperature effects and power dissipation 33 // 2.5 Complexation equilibrium 36 // 2.6 Electric field-mediated migration of biopolymers in sieving matrices 41 // 2.7 Micropreparative capillary gel electrophoresis 47 // References 51 // 3. Separation matrix and column technology 57 // 3.1 Gels and polymer networks 57 // 3.1.1 Cross-linked (chemical) gels 61 // 3.1,2 Noncross-linked polymer solutions (physical gels) 70 // 3.2 Alternative matrices (composite gels, pluronics, agarose, sol-gel systems, block polymers) 84 // 3.2.1 Agarose-based gel compositions 90 // 3.2.2 Other alternative matrices 92 // 3.3 Capillary coatings 99 // 3.3.1 Covalent (chemical) coatings 104 // 3.3.2 Physical (noncovalent) surface coverage 107 // 3.4 Techniques for preparing gel-filled capillaries 110 // References 116 // 4. Instrumentation 129 // 4.1 Sample introduction methods 129 // 4.1.1 Electrokinetic injection into high-viscosity gels 132 // 4.1.2 Hydrodynamic injection into low-viscosity polymer solutions 135 // 4.1.3 Sample preconcentration 136 // 4.1.4 Effect of sample overloading 145 //

4.1.5 Injection-related artifacts 146 // 4.2 Detection systems 146 // 4.2.1 UV absorbance and diode array detection 151 // 4.2.2 Laser-induced fluorescence detection 153 // 4.2.3 Indirect detection methods 164 // 4.2.4 Electrochemical and conductivity detection 165 // 4.2.5 Miscellaneous detector systems 166 // 4.2.6 Coupling to mass spectrometers 170 // 4.3 Operation variables 176 // 4.3.1 Gel concentration 176 // 4.3.2 Separation voltage and temperature 179 // 4.3.3 Capillary dimensions 182 // 4.3.4 Buffer systems 183 // 4.3.5 Nonaqueous electrophoresis, organic modifiers 183 // References 185 // 5. Applications 199 // 5.1 Capillary gel electrophoresis of DNA 199 // 5.1.1 Analysis of single-stranded oligonucleotides 199 // 5.1.2 dsDNA fragments and PCR products 223 // 5.1.3 Large chromosomal DNA and pulsed-field electrophoresis 241 // 5.1.4 Antisense DNA and modified oligonucleotides 254 // 5.1.5 Biomedical and forensic applications 256 // 5.2 Capillary gel electrophoresis of proteins 265 // 5.2.1 Capillary SDS gel electrophoresis 266 // 5.2.2 Ultrafast separations 276 // 5.2.3 Capillary gel isoelectric focusing of proteins 278 // 5.2.4 Fluorescent labeling of proteins for SDS-CGE 282 // 5.2.5 Applications in biotechnology 289 // 5.3 Capillary gel electrophoresis of carbohydrates 298 // 5.3.1 Analytical glycobiology 299 // 5.3.2 Release of N- and 0-linked oligosaccharides from glycoproteins 299 // 5.3.3 Fluorophore labeling of carbohydrates 300 // 5.3.4 Capillary gel electrophoresis separation of oligosaccharides 300 // 5.3.5 Selected other applications 308 // 5.4 Capillary affinity gel electrophoresis 310 // 5.4.1 Chiral affinity additives and chiral selectors 312 // 5.4.2 Capillary affinity gel electrophoresis of DNA 316 // 5.4.3 Other capillary affinity gel electrophoresis applications 320 // References 324 //

6. Related microseparation techniques 351 // 6.1 Ultrathin-layer gel electrophoresis 351 // 6.1.1 DNA analysis 351 // 6.1.2 Protein analysis 356 // 6.2 Multidimensional approaches 358 // References 361 // 7. Appendix 365 // Manufacturers’ directory 365 // Further reading 366 // Abbreviations // Index

(OCoLC)1289812510